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TP4063:Rao-Larkin酒精液體模型飼料
Rao-Larkin Ethanol Liquid Diet(26% ethanol, TP4063)

一、Rao-Larkin ethanol liquid diet(26% ethanol)介紹


Rao的課題組針對Lieber-DeCarli標準型(regular型)酒精液體模型飼料進行了大量研究,提出了大量的證據證明標準型Lieber-DeCarli酒精液體飼料引起的脂肪肝與營養不良有關,關鍵原因是,酒精導致大鼠厭食癥,從而攝入營養素不足,最為有力的證據是:

(1)關于脂肪肝的觀察

用36%酒精喂養造成脂肪肝的動物改用26%酒精液體飼料,脂肪肝消退或減輕,反過來,26%酒精液體飼料喂養后的動物沒有出現脂肪肝,此時轉為喂養36%酒精時,動物出現脂肪肝。

(2)動物的酒精攝入量和血液酒精濃度

比起36%酒精液體飼料的喂養,26%酒精液體飼料喂養時動物攝入飼料量要多(熱量攝入要高),酒精攝入量要多,但血液酒精濃度卻是降低。

(3)與顆粒型(棒狀)標準飼料比較

與顆粒型標準飼料喂養的動物比較,36%酒精液體飼料喂養后,體重增加速度較小。

他們認為,動物屬于營養素缺乏狀態可以介紹以上(2)和(3),而(1)中36%酒精液體飼料引起的所謂酒精性脂肪脂肪肝實際上由于酒精引起厭食癥,使得攝入熱量不足,并且因為攝入量過低導致營養素攝入量不足,從而出現了營養素缺乏,引起肝臟代謝發生改變和脂肪肝,而血液酒精濃度之所以要比26%酒精液體飼料要高的原因正是因為營養素相對不足所致酒精分解代謝減少。

此外,他們認為,除了酒精性脂肪肝模型之外,其他方面的研究不應該采用36%酒精的高脂液體飼料,而應該改用26%的酒精水平。這就是這里所稱的Rao-Larkin酒精液體飼料。

Rao-Larkin酒精液體模型飼料
【1】Rao-Larkin酒精液體模型飼料:

脂肪35%, 蛋白質18%, 碳水化合物21%, 酒精26%。

南通特洛菲飼料科技有限公司模型飼料代碼:TP4063;

【2】Rao-Larkin酒精液體對照飼料:

脂肪35%, 蛋白質18%, 碳水化合物47%。

南通特洛菲飼料科技有限公司對照飼料代碼:TP4063C;

二、TP4063(Rao-Larkin)ethanol liquid diet的用途


從以上可知,TP4063適合于除了酒精性脂肪肝造模之外酒精的效應及其機制的研究。

Rao-Larkin酒精液體模型飼料的用途
廣泛應用于大鼠、小鼠、兔、地鼠等動物:

(1)酒精性肝損傷的復制;

(2)酒精性肝損傷模型的復制;

(3)酒精性胰腺炎模型的復制

(4)藥物、食品功能因子、保健食品對酒精性肝損傷的保護或對抗作用;

(5)藥物、食品功能因子、保健食品對酒精的解毒作用;

(6)酒精成癮的機制;

(7)酒精所致生理機能的改變及其機制。

(8)酒精與毒素(環境、食物、微生物的毒素)對機體的相互作用。

請注意動物不同種系、品系、年齡、性別的差異。

三、TP4063購買的注意點


關于Lieber-DeCarli酒精液體模型飼料的詳細介紹,請點擊“Lieber-DeCarli酒精液體飼料和注意事項”。

1.購買飼料前:

(1)應當做好動物的準備,注意動物的品種、品系、年齡、性別的選擇。

(2)準備好喂養流程的準備。

2. 購買后應當及時向公司索取以下資料:

《液體飼料配制方法》

《酒精液體飼料喂養方法》

《怎樣根據研究的需要調整酒精的配比》

 

References:

Samudio-Ruiz SL, Allan AM, Sheema S, Caldwell KK. Hippocampal N-methyl-D-aspartate receptor subunit expression profiles in a mouse model of prenatal alcohol exposure. Alcohol Clin Exp Res. 2010 Feb;34(2):342-53.

Chakkalakal DA, Novak JR, Fritz ED, Mollner TJ, McVicker DL, Garvin KL, McGuire MH, Donohue TM. Inhibition of bone repair in a rat model for chronic and excessive alcohol consumption. Alcohol. 2005 Jul;36(3):201-14.

Wyatt TA, Gentry-Nielsen MJ, Pavlik JA, Sisson JH. Desensitization of PKA-stimulated ciliary beat frequency in an ethanol-fed rat model of cigarette smoke exposure. Alcohol Clin Exp Res. 2004 Jul;28(7):998-1004.

Gentry-Nielsen MJ, Top EV, Snitily MU, Casey CA, Preheim LC. A rat model to determine the biomedical consequences of concurrent ethanol ingestion and cigarette smoke exposure. Alcohol Clin Exp Res. 2004 Jul;28(7):1120-8.

Frishman WH, Del Vecchio A, Sanal S, Ismail A. Cardiovascular manifestations of substance abuse: part 2: alcohol, amphetamines, heroin, cannabis, and caffeine. Heart Dis. 2003 Jul-Aug;5(4):253-71.

Costa ET, Olivera DS, Meyer DA, Ferreira VM, Soto EE, Frausto S, Savage DD, Browning MD, Valenzuela CF. Fetal alcohol exposure alters neurosteroid modulation of hippocampal N-methyl-D-aspartate receptors. J Biol Chem. 2000 Dec 8;275(49):38268-74.

Costa ET, Olivera DS, Meyer DA, Ferreira VM, Soto EE, Frausto S, Savage DD, Browning MD, Valenzuela CF. Fetal alcohol exposure alters neurosteroid modulation of hippocampal N-methyl-D-aspartate receptors. J Biol Chem. 2000 Dec 8;275(49):38268-74.

Rao GA, Larkin EC. Nutritional factors required for alcoholic liver disease in rats. J Nutr. 1997 May;127(5 Suppl):896S-898S.

Allan AM, Wu H, Paxton LL, Savage DD. Prenatal ethanol exposure alters the modulation of the gamma-aminobutyric acidA1 receptor-gated chloride ion channel in adult rat offspring. J Pharmacol Exp Ther. 1998 Jan;284(1):250-7.

Sankaran H, Deveney CW, Larkin EC, Rao GA. Carbohydrate intake determines pancreatic acinar amylase activity and release despite chronic alcoholemia in rats. J Nutr. 1992 Sep;122(9):1884-91.

Derr RF, Draves K. Prevention of alcohol toxicity by adequate nutrients: mechanism and potential application. Nutr Res 1990, 10(11):1285-1297.

López JM, Bombi JA, Valderrama R, Giménez A, Parés A, Caballería J, Imperial S, Navarro S. Effects of prolonged ethanol intake and malnutrition on rat pancreas. Gut. 1996 Feb;38(2):285-92.

Derr RF. The quantities of nutrients recommended by the NRC abate the effects of a toxic alcohol dose administered to rats. J Nutr. 1989 Aug;119(8):1228-30.

Preedy VR, Duane P, Peters TJ. Biological effects of chronic ethanol consumption: a reappraisal of the Lieber-De Carli liquid-diet model with reference to skeletal muscle. Alcohol Alcohol. 1988;23(2):151-4.

Rao GA, Larkin EC. Inadequate intake by growing rats of essential nutrients from liquid diets used for chronic alcohol consumption. Nutr Res, 1985; 5(7):789-796.

Rao GA, Riley DE, Larkin E. Lieber-decarli alcohol diet modification to enhance growth in young rats. Nutr Res,1986,6(1):101–105.

Nguyen T, Chi CW, Larkin EC, Rao GA. Low liver glycogen content in alcoholic rats due to depressed carbohydrate ingestion. Biochem Arch 1990 6(2):217-221 .

Sankaran H, Desai AJ, Deveney CW, Larkin EC, Rao GA. Energy restriction and chronic alcoholemia prolong the delay in gastric emptying in rats. Nutr Res,1996, 16(5):855-864.

Sankaran H, Deveney CW, Larkin EC, Rao GA. Carbohydrate intake determines pancreatic acinar amylase activity and release despite chronic alcoholemia in rats. J. Nutr. 1992, 122: 1884–1891.

Rao GA, Larkin EC, Derr RF.Chronic alcohol consumption during pregnancy: alleviation of untoward effects by adequate nutrition. Nutr Res, 1988,8(4):421–429.



有困惑?那就商量唄!

液體飼料的優點,你可以充分運用!

---------《》-------

marker可以任意缺乏或過載某營養素

marker可以任意添加藥物或測試成分

marker可以精確定量飼料攝入量

marker可以任意定制飼料


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