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TP4062:Tipoe-Nanji酒精液體模型飼料(29%酒精型)
Tipoe-Nanji Ethanol Liquid Diet(Nanji diet,29%ethanol)

一、Tipoe-Nanji diet with 29% alcohol的由來和特點


特洛菲飼料科技有限公司
Tipoe-Nanji-29%酒精液體飼料
【1】模型飼料:

南通特洛菲飼料科技有限公司飼料代碼:TP4062;

脂肪35%, 蛋白質36%, 酒精29%(4.5% v/v)。

配制1升(1000毫升)需要飼料149.8g,魚油41.8g,添加95%酒精或無水酒精。配成液體后每毫升1千卡。

【2】對照飼料:

南通特洛菲飼料科技有限公司飼料代碼:TP4062C;

脂肪35%, 蛋白質36%, 碳水化合物29%。

配制1升(1000毫升)需要飼料178.2g,魚油41.8g,添加95%酒精或無水酒精。配成液體后每毫升1千卡。

Tipoe-Nanji diet當前有兩種常用的酒精液體模型飼料,一種是酒精占40%的酒精液體飼料,另一種是下面要介紹的酒精占29%的酒精液體飼料,兩種模型飼料都是以魚油作為主要脂肪原料。40%酒精液體飼料首先被創建(務必點擊了解),然后出現了29%酒精液體飼料。

文獻中把這兩種飼料都成為Nanji diet,而實際上,從相關文獻分析,Tipoe對這兩種模型飼料的貢獻起了重要作用,因此,我們這里稱為Tipoe-Nanji diet。

毫無疑問,29%酒精的Tipoe-Nanji酒精液體飼料提供的酒精要比40%酒精的要低得多。然而,在喂養大鼠和小鼠的效果方面,目前還沒有資料說明有什么差別,比如,都能引起酒精性脂肪肝,腸粘膜通透性增加,內毒素血癥,等等。

盡管如此,效應的差異性一定是有的,因為兩種飼料之間有兩個最為關鍵的區別,一是,29%酒精型幾乎沒有碳水化合物。以往的研究中已經知道,如果酒精液體飼料中碳水化合物過低時,肝臟糖代謝異常,ATP生成減少(減慢)。二是,29%酒精型酒精含量低,能夠推測到血液酒精水平可能較低,這就可以推測作用比較溫和,因此,特別適合慢性肝內、肝外組織或器官的毒性研究。

然而,29%酒精型的優勢正是因為酒精含量低,使得動物容易接受。試想,魚油的味道是難以接受的,再讓動物飲酒,確實讓為我們的研究做出犧牲的大小鼠們為難了。

Tipoe-Nanji 29%酒精液體模型飼料的用途和特點
Tipoe-Nanji酒精液體模型飼料的用途:主要是:

【1】酒精性肝病、酒精性骨質疏松癥等疾病的造模和機制的研究;

【2】在酒精性肝病造模中,不僅有脂肪肝,而且可伴有炎癥、壞死、ALT升高,內毒素血癥;

【2】藥物、食品功能因子、保健品對酒精或酒精性疾病的拮抗作用;

【3】飲食不同脂肪或不同脂肪酸構成與酒精在酒精毒性或酒精性疾病中的相互作用的研究;

Tipoe-Nanji酒精液體模型飼料的特點:

【1】高脂肪、低碳水化合物;

【2】脂肪以魚油為主;

【3】自由喂養。

 

二、Tipoe-Nanji diet with 29% alcoho應用的注意點


1.購買飼料前:

(1)由于含量大量的魚油,應當準備4度存放飼料的條件。

(2)應當做好動物的準備,注意動物的月齡、體重,特別注意動物的品種和品系的選擇。

(3)務必熟知飼料喂養流程。

2. 購買手續完成后應當向公司索取以下資料:

《液體飼料配制方法》

《酒精液體飼料喂養方法》

《怎樣根據研究的需要調整酒精的配比》

References:

Tipoe GL, Ho CT, Liong EC, Leung TM, Lau TY, Fung ML, Nanji AA. Voluntary oral feeding of rats not requiring a very high fat diet is a clinically relevant animal model of non-alcoholic fatty liver disease (NAFLD). Histol Histopathol. 2009 Sep;24(9):1161-9.

Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Thomas P, Dannenberg AJ. Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am J Physiol Gastrointest Liver Physiol. 2003 Feb;284(2):G321-7.

Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Dannenberg AJ. Dietary saturated fatty acids reverse inflammatory and fibrotic changes in rat liver despite continued ethanol administration. J Pharmacol Exp Ther. 2001 Nov;299(2):638-44.

Summa KC, Voigt RM, Forsyth CB, Shaikh M, Cavanaugh K, Tang Y, Vitaterna MH, Song S, Turek FW, Keshavarzian A. Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation. PLoS One. 2013 Jun 18;8(6):e67102. Print 2013.

Tipoe GL, Liong EC, Casey CA, Donohue TM Jr, Eagon PK, So H, Leung TM, Fogt F, Nanji AA. A voluntary oral ethanol-feeding rat model associated with necroinflammatory liver injury. Alcohol Clin Exp Res. 2008 Apr;32(4):669-82.

Forsyth CB, Tang Y, Shaikh M, Zhang L, Keshavarzian A (2011) Role of snail activation in alcohol-induced iNOS-mediated disruption of intestinal epithelial cell permeability. Alcohol Clin Exp Res 35: 1635–1643.

Nanji AA, Zhao S, Sadrzadeh SM, Dannenberg AJ, Tahan SR, Waxman DJ. Markedly enhanced cytochrome P450 2E1 induction and lipid peroxidation is associated with severe liver injury in fish oil-ethanol-fed rats. Alcohol Clin Exp Res. 1994 Oct;18(5):1280-5.

Morimoto M, Zern MA, Hagbj?rk AL, Ingelman-Sundberg M, French SW. Fish oil, alcohol, and liver pathology: role of cytochrome P450 2E1. Proc Soc Exp Biol Med. 1994 Nov;207(2):197-205.

Nanji AA, Zhao S, Lamb RG, Sadrzadeh SM, Dannenberg AJ, Waxman DJ. Changes in microsomal phospholipases and arachidonic acid in experimental alcoholic liver injury: relationship to cytochrome P-450 2E1 induction and conjugated diene formation. Alcohol Clin Exp Res. 1993 Jun;17(3):598-603.

Nanji AA, Zhao S, Lamb RG, Dannenberg AJ, Sadrzadeh SM, Waxman DJ. Changes in cytochromes P-450, 2E1, 2B1, and 4A, and phospholipases A and C in the intragastric feeding rat model for alcoholic liver disease: relationship to dietary fats and pathologic liver injury. Alcohol Clin Exp Res. 1994 Aug;18(4):902-8.

Fogt F, Nanji AA. Alterations in nuclear ploidy and cell phase distribution of rat liver cells in experimental alcoholic liver disease: relationship to antioxidant enzyme gene expression.Toxicol Appl Pharmacol. 1996 Jan;136(1):87-93.

Polavarapu R, Spitz DR, Sim JE, Follansbee MH, Oberley LW, Rahemtulla A, Nanji AA. Increased lipid peroxidation and impaired antioxidant enzyme function is associated with pathological liver injury in experimental alcoholic liver disease in rats fed diets high in corn oil and fish oil. Hepatology. 1998 May;27(5):1317-23.

Nanji AA, Fogt F, Griniuviene B. Alterations in glucose transporter proteins in alcoholic liver disease in the rat. Am J Pathol. 1995 Feb;146(2):329-34.

Li CJ, Nanji AA, Siakotos AN, Lin RC. Acetaldehyde-modified and 4-hydroxynonenal-modified proteins in the livers of rats with alcoholic liver disease. Hepatology. 1997 Sep;26(3):650-7.

Nanji AA, Dannenberg AJ, Jokelainen K, Bass NM. Alcoholic liver injury in the rat is associated with reduced expression of peroxisome proliferator-alpha (PPARalpha)-regulated genes and is ameliorated by PPARalpha activation. J Pharmacol Exp Ther. 2004 Jul;310(1):417-24.

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Wimberly AL, Forsyth CB, Khan MW, Pemberton A, Khazaie K, Keshavarzian A. Ethanol-induced mast cell-mediated inflammation leads to increased susceptibility of intestinal tumorigenesis in the APC Δ468 min mouse model of colon cancer. Alcohol Clin Exp Res. 2013 Jan;37 Suppl 1:E199-208.

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Morimoto M, Zern MA, Hagbj?rk AL, Ingelman-Sundberg M, French SW. Fish oil, alcohol, and liver pathology: role of cytochrome P450 2E1. Proc Soc Exp Biol Med. 1994 Nov;207(2):197-205.

Tsukamoto H, Towner SJ, Ciofalo LM, French SW. Ethanol-induced fibrosis rats fed high fat diet. Hepatology 1986, 6:814-822

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Nanji AA, French SW. Dietary factors and alcoholic cirrhosis.Alcohol Clin Exp Res 1986, 10:271-273

Nanji AA, French SW. Relationship between pork consumption and cirrhosis. Lancet 1985, 1:681-683

Nanji AA, Mendenhall CL, French SW. Beef fat prevents alcoholic liver disease in the rat. Alcohol Clin Exp Res 1989, 13:15-19

Nanji AA, French SW. Dietary linoleic acid is required for development of experimental alcoholic liver disease. Life Sci 1989, 44:223-227

French SW: Nutrition in the pathogenesis of alcoholic liver disease. Alcohol Alcoholism 1993, 28:97-109 French SW. Miyamoto K, Tsukamoto H: Ethanol-induced fibrosis in the rat: role of amount of dietary fat. Alcohol Clin Exp Res 1986, 10:13S-19S

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有困惑?那就商量唄!

液體飼料的優點,你可以充分運用!

---------《》-------

marker可以任意缺乏或過載某營養素

marker可以任意添加藥物或測試成分

marker可以精確定量飼料攝入量

marker可以任意定制飼料


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