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動脈粥樣硬化模型


非人靈長類(猴,猩猩,狒狒,等)動脈粥樣硬化模型飼料
和造模注意事項
Model Diet of Atherosclerosis for non-human primate (monkey or orangutan etc)

非人靈長類動物(猴子、猩猩,等)在種系上最為接近人類,研究結果更能推演到人類。如果你準備采用這類動物,提醒你要特別注意倫理方面的規定。

一、猴或狒狒動脈粥樣硬化模型造模的特點


猴或狒狒作為人類動脈粥樣硬化模型動物,具有以下方面的表現:

(1)猴的血脂代謝接近人類,血漿膽固醇主要在LDL中。

(2)血漿脂蛋白對模型飼料的反應與人類相似,模型飼料導致的血脂異常與動脈粥樣硬化高度關聯。

(3)動脈粥樣硬化容易發生,甚至可以自發發生。

(4)模型飼料誘導的動脈粥樣硬化,發生于主動脈及其所有大的分支(如,冠狀動脈和顱內腦動脈),可以發展到動脈粥樣硬化高級階段,并且容易發生致命性心肌梗塞,這些都是人類發生動脈粥樣硬化的類似表現。

二、動脈粥樣硬化模型造模中猴或狒狒的選擇和使用方法


猴作為人類動脈粥樣硬化的模型動物開展研究,需要特別注意猴的選擇和使用方法:

1.一般使用舊大陸猴(old world monkey),不使用新大陸猴(New World monkeys)

舊大陸猴包括猴科成員(長尾猴、狒狒等),也可以包括長臂猿科(Hylobatidae)、猩猩科(Pongidae)。

常用于動脈粥樣硬化模型的猴包括獼猴(恒菏猴,rhesus monkey)、松鼠猴(squirrel monkey),食蟹猴(cynomolgus monkey,cynomolgus macaques),卷尾猴(Cebus albifrons),也有使用伶猴(titi)。

新大陸猴(例如,卷尾猴,闊鼻猴),由于動脈粥樣硬化引起嚴重的慢性腎臟疾病,后者又影響血脂代謝和動脈粥樣硬化,因此,動物容易發生嚴重病態甚至死亡,因此,這些動物不應當用于動脈粥樣硬化模型的造模。豬能夠自發發生動脈粥樣硬化,對照喂養的豬正常飼料要求較高,需要使用符合要求的普通飼料。

2. 不同種類的猴或狒狒之間,或者相同猴之間,對動脈粥樣硬化的敏感程度或者反應性不同,需要進行敏感性(反應性)測試

即便是相同的猴,相同的性別,不同個體對動脈粥樣硬化的反應性不同,又由于每組動物數不可能大,因此,應當避免因為個體之間反應性不同而導致的統計學分析結果的不確定性。

為此,可采取的方法是,需要應用模型飼料進行喂養試驗,觀察血漿膽固醇濃度的改變情況,根據動物的反應性分為高反應性組和低反應性組。或者,只選用高反應性的動物。

3. 動物的年齡和性別

除非研究中有特殊要求,一般使用成年、雄性的猴或狒狒。

三、猴或狒狒的動脈粥樣硬化模型造模用高脂高膽固醇模型飼料


下表列出的是南通特洛菲飼料科技有限公司提供的豬或微型豬動脈粥樣硬化模型飼料。

特洛菲飼料科技有限公司
猴或或狒狒高脂高膽固醇模型飼料

日糧型

以下模型飼料是在本公司豬標準飼料(低脂)生產過程中添加脂肪和膽固醇,其正常對照飼料應當選用TP 2R001。

產品代碼     模型飼料名稱                             說明

TP3M310:非人靈長類高脂高膽固醇模型飼料,38%(熱量)脂肪,1%膽固醇

TP3M320:非人靈長類高脂高膽固醇模型飼料,43%(熱量)脂肪,0.7%膽固醇

TP3M330:非人靈長類高脂高膽固醇模型飼料,45%(熱量)脂肪,0.7%膽固醇

如果以上模型飼料不能滿足你的要求,請提出!

純化型

以下模型飼料是純化日糧,價格相對較高。

產品代碼    模型飼料名稱                    說明

TP3M700:非人靈長類高脂高膽固醇飼料,18%脂肪,0.08%膽固醇

TP3M710:非人靈長類高脂高膽固醇飼料,25%脂肪,0.2%膽固醇

如果以上模型飼料不能滿足你的要求,請提出!

對于上述描述中有不清楚之處或者其他注意事項,請與南通特洛菲飼料科技有限公司技術部聯系。

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