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動脈粥樣硬化模型


 Golden Hamster(金黃地鼠)

金黃地鼠動脈粥樣硬化模型飼料
和造模注意事項
Model Diet of Atherosclerosis in Golden Hamster

金黃地鼠歸類于小型嚙齒動物,用于研究血脂和動脈粥樣硬化的地鼠一般都是金黃地鼠(golden hamster)。地鼠的血脂代謝與大鼠、小鼠有較大程度不同,包括擁有CETP、血漿膽固醇將近一半在LDL中,相比較而言,地鼠血脂接近人類。除外,地鼠試驗容易。因此,豚鼠被常用于動脈粥樣硬化研究或者造模。

一、金黃地鼠動脈粥樣硬化模型的特點


1.金黃地鼠用于動脈粥樣硬化研究的優點:

(1)金黃地鼠血脂與人類比較接近。

(2)能夠發生動脈粥樣硬化,可以到達動脈粥樣硬化的早期(脂質條紋期,內皮下泡沫細胞)。

(3)動物小而溫順,容易喂養。

2.金黃地鼠用于動脈粥樣硬化研究的缺點:

(1)金黃地鼠有多種品系,不同品系之間動脈粥樣硬化模型的難易程度不同,相比較而言,F1B品系最理想。

(2)血漿HDL濃度比較高,對抗動脈粥樣硬化;

(3)血漿膽固醇升高既引起動脈粥樣硬化,又因為發揮抗氧化作用而削弱動脈粥樣硬化進程和程度。

正是這些缺點,金黃地鼠動脈粥樣硬化模型的復制方法與其他動物有所不同。

二、金黃地鼠動脈粥樣硬化造模方法和造模飼料


高脂高膽固醇模型飼料和對高膽固醇飼料兩種飼料都可以引起金黃地鼠動脈粥樣硬化(位于升主動和主動脈弓)。但是,程度不同:高膽固醇模型飼料可能不會引起動脈粥樣硬化,或者動脈粥樣硬化程度非常輕微。因此,在進行動脈粥樣硬化造模時,一般不用高膽固醇模型飼料造模,只有在研究高膽固醇對動脈粥樣硬化形成的影響和機制時采用高膽固醇模型飼料。

所以,下面先介紹動脈粥樣硬化造模時高脂高膽固醇模型飼料的使用和造模方法注意事項,然后怎樣觀察高膽固醇的效應。

1.金黃地鼠用于動脈粥樣硬化造模的高脂高膽固醇模型飼料

金黃地鼠發生動脈粥樣硬化的程度對于高脂高膽固醇模型飼料的要求比較高,關鍵在于對脂肪類型和脂肪酸的構成有較高要求,而主要不在于脂肪含量和膽固醇含量的高低。

飼料中脂肪含量高低取決于研究中是否需要伴有肥胖。

特洛菲飼料科技有限公司
金黃地鼠高脂高膽固醇模型飼料

日糧型

以下模型飼料是在本公司標準飼料(低脂)生產過程中添加脂肪和膽固醇,其正常對照飼料應當選用相應的低脂飼料。

產品代碼     模型飼料名稱                          說明

TP 3H303:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,0.2%膽固醇

TP 3H305:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,0.3%膽固醇

TP 3H307:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,0.5%膽固醇

TP 3H310:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,1%膽固醇

TP 3H320:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,2%膽固醇

TP 3H330:金黃地鼠高脂高膽固醇模型飼料,15%脂肪,3%膽固醇

TP 3H340:金黃地鼠高脂高膽固醇模型飼料,17%脂肪,0.1%膽固醇

TP 3H350:金黃地鼠高脂高膽固醇模型飼料,25%脂肪,2%膽固醇

TP 3H360:金黃地鼠高脂高膽固醇模型飼料,30%脂肪,0.2%膽固醇

TP 3H370:金黃地鼠高脂高膽固醇模型飼料,40%脂肪,0.2%膽固醇

如果以上模型飼料不能滿足你的要求,請提出!

純化型

以下模型飼料是純化日糧,價格相對較高。

產品代碼     模型飼料名稱                          說明

TP 3H405:金黃地鼠高脂高膽固醇模型飼料,10%脂肪,0.2%膽固醇

TP 3H420:金黃地鼠高脂高膽固醇模型飼料,17%脂肪,0.1%膽固醇

TP 3H440:金黃地鼠高脂高膽固醇模型飼料,25%脂肪,2%膽固醇

如果以上模型飼料不能滿足你的要求,請提出!

2.金黃地鼠用于動脈粥樣硬化發展過程中膽固醇的作用的研究方法和高脂高膽固醇模型飼料

由于高膽固醇飼料喂養金黃地鼠的效果至今沒有一致的效果,因此,研究高膽固醇飲食對金黃地鼠動脈粥樣硬化的作用和原因至今不清楚。

而可行的方法是,為了研究膽固醇對動脈粥樣硬化的作用,可以采用高脂高膽固醇模型飼料與高脂模型飼料的喂養效果進行對比。但是,這其中要注意:

(1)高脂肪飼料喂養也能引起金黃地鼠動脈粥樣硬化,并且脂肪類型或者脂肪中脂肪酸的構成比例對動脈粥樣硬化有區別。

(2)高脂飼料喂養引起的血脂改變(HDL的改變和nonHDL的改變)個體間差異較大。

(3)在喂高脂高膽固醇模型飼料的金黃地鼠中,只有血漿中nonHDL(即LDL+VLDL)處于高濃度區的金黃地鼠中表現出高膽固醇模型飼料對動脈粥樣硬化的促進作用,在低濃度區nonHDL的金黃地鼠中表現為拮抗作用,而在nonHDL濃度處于中間段的動物沒有作用。這個濃度區間的界定,要看動物的具體情況。

對于上述描述中有不清楚之處或者其他注意事項,請與南通特洛菲飼料科技有限公司技術部聯系。

References:

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