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小鼠肝臟干細胞(卵圓細胞)誘導法(DDC模型飼料)
DDC-Induced Oval Cells from Mouse Liver

該法是在飼料中添加DDC(3,5-diethoxycarbonyl-1,4-dihydro-collidi?ne),DDC損傷肝細胞引起肝臟干細胞HSC(Quiescent hepatic stem cells)增加,從而通過分離、純化、鑒定而獲得HSC。

【飼料代碼】LAD0011DDC,含0.1%DDC的模型飼料。

【實驗動物】小鼠。

【喂養方法】自由飲食。

【注意事項】DDC有強力的毒性,定制過程、運輸、使用過程中應當采取有效的防護措施。



參考文獻

Endo Y, Zhang M, Yamaji S, Cang Y. Genetic Abolishment of Hepatocyte Proliferation Activates Hepatic Stem Cells. PLoS ONE 2012,7(2): e31846. 期刊全文

Dorrell C, Erker L, Schug J, Kopp JL, Canaday PS, Fox AJ, Smirnova O, Duncan AW, Finegold MJ, Sander M, Kaestner KH, Grompe M. Prospective isolation of a bipotential clonogenic liver progenitor cell in adult mice. Genes Dev. 2011 Jun 1;25(11):1193-203. 期刊全文

Tanaka M, Miyajima A. Identification and isolation of adult liver stem/progenitor cells.Methods Mol Biol. 2012;826:25-32.

Yovchev, M. I., Grozdanov, P. N., Zhou, H., Racherla, H., Guha, C. and Dabeva, M. D. (2008) Identification of adult hepatic progenitor cells capable of repopulating injured rat liver. Hepatology 47, 636–647.

Suzuki, A., Sekiya, S., Onishi, M., Oshima, N., Kiyonari, H., Nakauchi, H., et al.  Flow cytometric isolation and clonal identification of self-renewing bipotent hepatic progenitor cells in adult mouse liver. Hepatology 2008,48, 1964–1978.

Okabe, M., Tsukahara, Y., Tanaka, M., Suzuki, K., Saito, S., Kamiya, Y., et al.  Potential hepatic stem cells reside in EpCAM+ cells of normal and injured mouse liver. Development 2009,136, 1951–1960.

Kamiya, A., Kakinuma, S., Yamazaki, Y. and Nakauchi, H. Enrichment and clonal culture of progenitor cells during mouse postnatal liver development in mice. Gastroenterology 2009,137, 1114–1126, 1126 e1–14.


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