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乙硫氨酸(DL-Ethionine)
作為工具藥的模型及飼料

大鼠肝臟干細胞(卵圓細胞)誘導法(CDE模型飼料)
Choline-Deficient Ethionine-Supplemented (CDE)Diet-Induced Oval Cells from Rat Liver

大鼠是最先采用CDE模型飼料誘導肝臟干細胞( hepatic stem/progenitor cells)的動物。該法采用乙硫氨酸(DL-ethionine)損傷肝細胞,引起的損傷性反應中包括干細胞的反應性增加,從而通過分離、純化、鑒定而獲得肝臟的干細胞。

文獻中一般采用以下3種方法之一:

南通特洛菲飼料科技有限公司
大鼠CDE模型飼料
模型飼料代碼 方法說明
TP 0100GC50E CDE中含乙硫氨酸0.05%,直接喂養。
TP 0100GC50E01 CDE中含乙硫氨酸0.1%,直接喂養。
TP 0100GC50E05 CDE中含乙硫氨酸0.5%,直接喂養。
TP 0100GC50 飲水中添加乙硫氨酸;或乙硫氨酸灌胃
TP 1R806CD
TP 36104

(1)含0.1%乙硫氨酸的膽堿缺乏飼料(CDE模型飼料)

(2)膽堿缺乏飼料,飲水中加乙硫氨酸

乙硫氨酸一般用0.1%, 0.15%,。

(3)膽堿缺乏飼料,灌胃。

劑量是:按照動物每天飼料飼料量計算,相當于飼料量的0.05~0.1%,或者每天每kg體重50-70mg。(Kaplanski,等)

【乙硫氨酸】Sigma公司 No #E5139;Acros公司,等。

【實驗動物】大鼠,年齡不限,但最常用成年或接近成年。

【喂養時間】6周左右。

【注意事項】

 


參考文獻

Carpentier R1, Su?er RE, van Hul N, Kopp JL, Beaudry JB, Cordi S, Antoniou A, Raynaud P, Lepreux S, Jacquemin P, Leclercq IA, Sander M, Lemaigre FP.  Embryonic ductal plate cells give rise to cholangiocytes, periportal hepatocytes, and adult liver progenitor cells. Gastroenterology. 2011 Oct;141(4):1432-8, 1438.e1-4. 【Pubmed

Plenat F1, Braun L, Fausto N. Demonstration of glucose-6-phosphatase and peroxisomal catalase activity by ultrastructural cytochemistry in oval cells from livers of carcinogen-treated rats.Am J Pathol. 1988 Jan;130(1):91-102. 【Pubmed

Kaplanski C, Pauley CJ, Griffiths TG, Kawabata TT, Ledwith BJ.  Differentiation of rat oval cells after activation of peroxisome proliferator-activated receptor alpha43. Cancer Res. 2000 Feb 1;60(3):580-7.期刊全文

Shinozuka H., Lombardi B., Sell S., Iammarino R. M. Early histological and functional alterations of ethionine liver carcinogenesis in rats fed a choline-deficient diet. Cancer Res., 38: 1092-1098, 1978.

Habib SHM, Makpol S, Hamid NAA, Das S, Ngah WZW, Yusof YAM. Ginger extract (zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics. 2008;63:807-13.期刊全文

Tee LB, Kirilak Y, Huang WH, Morgan RH, Yeoh GC. Differentiation of oval cells into duct-like cells in preneoplastic liver of rats placed on a choline-deficient diet supplemented with ethionine. Carcinogenesis. 1994;15(12):2747–2756. 期刊全文

Guest I, Ilic Z, Sell S. Age dependence of oval cell responses and bile duct carcinomas in male fischer 344 rats fed a cyclic choline-deficient, ethionine-supplemented diet. .Hepatology. 2010 Nov;52(5):1750-7.  該期刊網站的全文

Hayner NT, Braun L, Yaswen P, Brooks M, Fausto N. Isozyme profiles of oval cells, parenchymal cells, and biliary cells isolated by centrifugal elutriation from normal and preneoplastic livers. Cancer Res. 1984 Jan;44(1):332-8. 該期刊網站的全文

Wojcik M, Bobowiec R, Lisiecka U, Kostro K. Proliferation, differentiation and apoptosis of choline deficient ethionine supplemented diet-rat oval cells under the influence of 2-methoxyestradiol. .J Physiol Pharmacol. 2012 Dec;63(6):669-76.期刊全文

Kubota K, Soeda J, Misawa R, Mihara M, Miwa S, Ise H, Takahashi M, Miyagawa S. Bone marrow-derived cells fuse with hepatic oval cells but are not involved in hepatic tumorigenesis in the choline-deficient ethionine-supplemented diet rat model. Carcinogenesis. 2008 Feb;29(2):448-54.

Steinberg P, Steinbrecher R, Schrenk D, Munzel P, Bruck M, Gschaidmaier H, Oesch F, Bock KW. Drug-metabolizing enzyme activities in freshly isolated oval cells and in an established oval cell line from carcinogen-fed rats.Cell Biol Toxicol. 1994 Feb;10(1):59-65. 期刊全文

Tanaka M, Itoh T, Tanimizu N, Miyajima A. Liver stem/progenitor cells: their characteristics and regulatory mechanisms.J Biochem. 2011 Mar;149(3):231-9. 期刊全文

Okano J, Shiota G, Matsumoto K, Yasui S, Kurimasa A, Hisatome I, Steinberg P, Murawaki Y. Hepatocyte growth factor exerts a proliferative effect on oval cells through the PI3K/AKT signaling pathway.Biochem Biophys Res Commun. 2003 Sep 19;309(2):298-304.

Kordes C, H?ussinger D. Hepatic stem cell niches. J Clin Invest. 2013 May;123(5):1874-80.期刊全文

Wang B, Jacob ST. Role of cancer stem cells in hepatocarcinogenesis.Genome Med. 2011 Feb 22;3(2):11. 期刊全文

 


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